Thursday, July 24, 2025

Appendix 04_DNA

This is an appendix to: What Happened to Chargaff's Rules?


Description of the following HTML table values:

AT variance: Chargaff Rule discrepancy?
CG variance: Chargaff Rule discrepancy?

Columns
Atom Count: sum of carbon,hydrogen,nitrogen and oxygen atoms
atom Percent: atom count ÷ atom count total
DNA const: percent of atoms in a perfect sequence
variation from Const: difference between atom percent and DNA const
atom count variation: approximate atom count difference
error percent: rounded variation from const
Plus 'N' uncert: change in error percent (error Percent × 'N' uncertainty)


Totals Columns
Atom Count Total: sum of carbon,hydrogen,nitrogen and oxygen atoms
atom Percent total: sum of carbon,hydrogen,nitrogen and oxygen atom percents
DNA const total: sum of carbon,hydrogen,nitrogen and oxygen atom consts
variation from Const: difference between carbon,hydrogen,nitrogen and oxygen atom percent and DNA consts
atom count variation: sum of carbon,hydrogen,nitrogen and oxygen atom count variation
error percent: atom count variation total ÷ atom count total

Link: NC_004914.3
Organism: Stx2 converting phage II DNA

Nucleotide Count: 62,706

'A' count: 16,043
'C' count: 14,498
'G' count: 16,793
'T' count: 15,372
'AT' variance: 671
'CG' variance: 2,295

Atom
Type
Atom
Count
Atom
Percent
DNA
Const
Variation
From Const
Atom Count
Variation
Error
Percent
Plus 'N'
uncert.
carbon 299,032 32.2098435 32.2033898 0.0064537 1,929.87 0.65 0.65%
hydrogen 328,902 35.4272518 35.5932203 0.1659685 54,587.38 16.60 16.60%
nitrogen 238,418 25.6808852 25.4237288 0.2571564 61,310.72 25.72 25.72%
oxygen 62,035 6.6820195 6.7796610 0.0976415 6,057.19 9.76 9.76%
Totals 928,387 100 100 0.5272202 123,883 13.34% 13.34%


Link: NC_003288.1
Organism: Staphylococcus phage phiETA DNA

Nucleotide Count: 43,081

'A' count: 15,885
'C' count: 6,651
'G' count: 8,613
'T' count: 11,932
'AT' variance: 3,953
'CG' variance: 1,962

Atom
Type
Atom
Count
Atom
Percent
DNA
Const
Variation
From Const
Atom Count
Variation
Error
Percent
Plus 'N'
uncert.
carbon 208,754 32.5402244 32.2033898 0.3368346 70,315.57 33.68 33.68%
hydrogen 227,337 35.4369114 35.5932203 0.1563089 35,534.80 15.63 15.63%
nitrogen 166,307 25.9236570 25.4237288 0.4999282 83,141.56 49.99 49.99%
oxygen 39,128 6.0992072 6.7796610 0.6804538 26,624.80 68.05 68.05%
Totals 641,526 100 100 1.6735256 215,614 33.61% 33.61%


Link: NC_004167.1
Organism: Bacteriophage phi-105 DNA

Nucleotide Count: 39,325

'A' count: 12,388
'C' count: 7,556
'G' count: 9,232
'T' count: 10,149
'AT' variance: 2,239
'CG' variance: 1,676

Atom
Type
Atom
Count
Atom
Percent
DNA
Const
Variation
From Const
Atom Count
Variation
Error
Percent
Plus 'N'
uncert.
carbon 189,069 32.3751060 32.2033898 0.1717162 32,466.20 17.17 17.17%
hydrogen 206,774 35.4068100 35.5932203 0.1864103 38,544.81 18.64 18.64%
nitrogen 151,066 25.8676872 25.4237288 0.4439584 67,067.02 44.40 44.40%
oxygen 37,086 6.3503968 6.7796610 0.4292642 15,919.69 42.93 42.93%
Totals 583,995 100 100 1.2313490 153,996 26.37% 26.37%


Link: NC_003278.1
Organism: Pseudomonas phage phiCTX DNA

Nucleotide Count: 35,580

'A' count: 7,316
'C' count: 11,906
'G' count: 10,375
'T' count: 5,983
'AT' variance: 1,333
'CG' variance: 1,531

Atom
Type
Atom
Count
Atom
Percent
DNA
Const
Variation
From Const
Atom Count
Variation
Error
Percent
Plus 'N'
uncert.
carbon 165,994 31.9057861 32.2033898 0.2976037 49,400.43 29.76 29.76%
hydrogen 183,883 35.3442394 35.5932203 0.2489809 45,783.36 24.90 24.90%
nitrogen 136,139 26.1673423 25.4237288 0.7436135 101,234.80 74.36 74.36%
oxygen 34,247 6.5826322 6.7796610 0.1970288 6,747.64 19.70 19.70%
Totals 520,263 100 100 1.4872269 203,164 39.05% 39.05%


Link: NC_002321.1
Organism: Staphylococcus phage PVL proviral
DNA

Nucleotide Count: 41,401

'A' count: 15,484
'C' count: 5,928
'G' count: 7,964
'T' count: 12,025
'AT' variance: 3,459
'CG' variance: 2,036

Atom
Type
Atom
Count
Atom
Percent
DNA
Const
Variation
From Const
Atom Count
Variation
Error
Percent
Plus 'N'
uncert.
carbon 201,077 32.5829697 32.2033898 0.3795799 76,324.78 37.96 37.96%
hydrogen 219,030 35.4921142 35.5932203 0.1011061 22,145.27 10.11 10.11%
nitrogen 159,074 25.7767090 25.4237288 0.3529802 56,149.98 35.30 35.30%
oxygen 37,942 6.1482071 6.7796610 0.6314539 23,958.62 63.15 63.15%
Totals 617,123 100 100 1.4651201 178,576 28.94% 28.94%


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